Influence of vitamin C on cytogencity and biochemical parameters in chlorpyrifos intoxicated rats

Document Type : Scientific and Research

Authors

1 Toxicology, Animal Health Research Institute, Tanta Branch

2 Biochem. Toxicology. &Feed deff. Dep. Animal Health Research Institute, Dokki

Abstract

This study is aimed to evaluate the effect of vitamin C on Chlorpyrifos induced cytogencity, some serum biochemical parameters and liver pathology in rats. A total of twenty albino rats weighing (180g -200g) were used for this study. Rats were divided into 4 groups each of 5 rats. First group was given Chlorpyrifos 12 mg/kg bw. Second group was given Vitamin C 200 mg/kg bw + Chlorpyrifos 12 mg/kg bw. Third group was given vitamin C only and fourth group was left as control group. All doses were orally administered daily for 14 successive days. At the end of the experimental period, blood samples were collected from each rat for biochemical analysis. Rats were humanely euthanized and femur from all rats was taken for cytogenicity and liver was collected for histopathological study. Oral administration of chlorpyrifos for 14 days in a dose of 12 mg/kg b .w, significantly increased the percentage of micronucleated polychromatic erythrocytes (MPCEs), ratio of polychromatic erythrocytes to normochromatic erythrocytes (PCE/NCE) .On other hand the administration of tested pesticide with vitamin c, reduce the frequencies of MPCEs and directed toward normal.
The PCE/NCE ratio is a measure for red blood cell proliferation which gave a sign of toxicity or damage of some organs of the body. The effect on the liver is one of the main toxic effects of this product as there was a significant decrease in the total protein and albumin synthesized by the liver and increase in the leakage enzymes namely AST , ALT plus increased levels of in cholesterol and triglyceride. Histopathology revealed vacuolar of hepatocytes with random hepatocyte necrosis and mononuclear cell infiltration. Administration of vitamin C improved serum biochemical parameters and alleviated degenerative necrotic damage in the sections of liver examined histologically.
In conclusion vitamin C has beneficial effects as it tends to dampen chlorpyrifos toxicity in rats

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