Ameliorating Effect of White Tea Extract against Acrylamide Induced DNA Damage and Biochemical Changes in Male Rats

Document Type : Scientific and Research

Authors

1 Biochemistry, Toxicology and Feed Deficiency Department, AHRI, Dokki,Giza

2 Biochemistry, Toxicology and Feed Deficiency Department, AHRI, Dokki,Giza.

3 microbiology Dep.- Faculty of medicine for girls – Al-Azhar university

Abstract

In this study, the protective effect of white tea (WT) aqueous extract against cytogenicity, DNA damage and some biochemical changes induced by acrylamide (ACR) in male albino rats was investigated. Six groups containing 5 rats each were used in this study. Group 1 served as a control. Groups 2 and 3 were given single daily oral doses of 10 and 20 mg /kg. b. wt. for 15 successive days. Group 4 permitted free access to solubilized WT (1.5%) as the sole drinking fluid. Groups 5 and 6 were given the same doses as group 2 and 3 in combination with solubilized WT. Rats received acrylamide in both tested concentrations significantly decreased the amount of DNA/gm of spleen and significantly increased in the percentage of MPCEs and PCE/NCE ratio as compared with the control group. Administration of acrylamide to rats significantly increased serum transaminases (AST and ALT), alkaline phosphatase (ALP), urea, creatinine and malondialdehyde in both doses (low and high), while it significantly decreased reduced glutathione. Administration of WT to intoxicated rats normalized all the tested parameters. Conclusion: Dietary intake and occupational exposure to acrylamide (ACR) must be restricted besides raising the public awareness to ACR hazards. The white tea extract exhibits a protective effect against ACR damage.

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Egyptian Journal of Chemistry and Environmental Health
Volume 2, Issue 2
November 2016
Pages 78-92

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