A total samples (616) were collected from bovine (100 calf`s fecal samples; 216 calf`s internal organs; 100 cow`s fecal samples and 200 cow`s internal organs), the animals showed profuse diarrhea. The samples were submitted for the isolation and identification of E.coli O157:H7. The identified isolates were examined for their susceptibility to 16 antimicrobial agents. Multiplex PCR was used for the detection of plasmid-mediated ampC β-lactamases (MAABLs) in isolated strains. E.coli O157:H7 isolated with rate of (2%) from the fecal sample of calves and cows. While only o.46% were isolated from internal organs of calves, meanwhile E.coli O157:H7 not isolated from cows samples. The isolates showed multidrug resistance ranged from 6%- 100%. AmpC plasmid mediated β lactamas genes were detected in all isolates of E.coli O157:H7, where 3 isolates showed presence CMY-7, BIL-1 at fragment 462bp, one isolate showed the presence of DHA-1, DHA-2 at fragment 405bp and one isolate showed MIR-1T and ACT-1 were detected at a of LAT-1 to LAT-4, CMY-2 to fragment 302bp. PCR remains the gold standard for detection of plasmid AmpC 𝛽-lactamases. This study may represent the first report of variants of MIR-1Tand ACT-1 𝛽-lactamase-producing E. coli in Egypt. Phenotypic methods alone may not reflect the true number of PMABLs producers. Genotypic methods need to be employed in national surveillance studies in Egypt.
A, S., W.A, G., El-Shafii, S., & Farag, A. (2016). Occurrence of Plasmid-mediated ampC β-lactamases (PMABLs) Resistance Genes among E. coli O157:H7 Isolated from Bovine Diarrhea. Egyptian Journal of Chemistry and Environmental Health, 2(2), 45-55. doi: 10.21608/ejceh.2016.246172
MLA
Shaimaa R A; Gad El-Said. W.A; Soumaya S.A El-Shafii; Azza N Farag. "Occurrence of Plasmid-mediated ampC β-lactamases (PMABLs) Resistance Genes among E. coli O157:H7 Isolated from Bovine Diarrhea". Egyptian Journal of Chemistry and Environmental Health, 2, 2, 2016, 45-55. doi: 10.21608/ejceh.2016.246172
HARVARD
A, S., W.A, G., El-Shafii, S., Farag, A. (2016). 'Occurrence of Plasmid-mediated ampC β-lactamases (PMABLs) Resistance Genes among E. coli O157:H7 Isolated from Bovine Diarrhea', Egyptian Journal of Chemistry and Environmental Health, 2(2), pp. 45-55. doi: 10.21608/ejceh.2016.246172
VANCOUVER
A, S., W.A, G., El-Shafii, S., Farag, A. Occurrence of Plasmid-mediated ampC β-lactamases (PMABLs) Resistance Genes among E. coli O157:H7 Isolated from Bovine Diarrhea. Egyptian Journal of Chemistry and Environmental Health, 2016; 2(2): 45-55. doi: 10.21608/ejceh.2016.246172
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