Effect of Jojoba Oil and Extra Virgin Olive Oil on Genetic Expressions and DNA Damage in Induced CCl4 Toxicity in Rats

Nassar, Mohamed H; Ibrahim, Ibrahim A; Sobhy, Hanan M; Saleh, Sherif Y; Elbeltagy, Marwa A

doi:10.21608/ejceh.2017.245785

Effect of Jojoba Oil and Extra Virgin Olive Oil on Genetic Expressions and DNA Damage in Induced CCl4 Toxicity in Rats

Document Type : Scientific and Research

Authors

¹ Biochem .Toxicol. &Feed Deff. Dep. - Animal Health Research Institute – Ismailia – Egypt

² Biochemistry dept. faculty of vet. Seuz canal university – Egypt

³ Biochem .Toxicol. &Feed Deff. Dep. - Animal Health Research Institute – Dokki – Egypt

10.21608/ejceh.2017.245785

Abstract

The present study was carried out to investigate the effects of jojoba oil and extra virgin olive oil in normal and in carbon tetrachloride (CCl4) induced genetic toxicity in male albino rats. A total of sixty male albino rats (120-140 g) were used in this study. Rats were divided into 6 groups of ten animals each. Group one was kept as a negative control and fed on basal diet only, while the second group kept as positive control and was fed on basal diet and injected with CCl4 to induce genetic and hepatic toxicity. The third group was fed on basal diet mixed with jojoba oil. The fourth group was fed on basal die mixed with extra virgin olive oil. The fifth group was fed on basal diet mixed with jojoba oil and injected with CCl4. The sixth group was fed on basal diet mixed with extra virgin olive oil and injected with CCl4. At the end of experimental period, blood samples were collected from each rat for biochemical analysis and rats were then sacrificed to elucidate the effect on genetic and hepatic toxicity.
Subcutaneous injection of CCl4 caused a significant increase in serum levels of AST, ALT, ALP, triglyceride, total cholesterol, LDL-c and lipid peroxidation (MDA) but it caused a significant decrease of HDL-c and reduced glutathione (GSH).The smear of hepatic DNA on agarose gel had been observed in CCl4 treated groups indicating random DNA fragmentation and a hallmark of necrosis. The genetic expression of hepatic CYP3A2 and GADD153 genes showed marked up regulation in CCl4 group.
Jojoba oil significantly restored the serum levels of biochemical parameters directed toward normal as compared with the positive control group except for ALT and genetic expression. The extra virgin olive oil showed better amelioration for biochemical and genetic parameters when compared with the positive control group.

Main Subjects