Hepato Protective Effect of Dried MUSHROOM against CCL4 Induced Hepato Toxicity in Liver and Cytogenicity Change in Rats

Document Type : Scientific and Research

Authors

1 Research Institute of Ophthalmology(RIO)

2 Biochem.Toxicol.&Food Deff. Dep. - Animal Health Research Institute

3 Nutrition and food science Dep.-Faculty of Home Economics Al –Azhar university

Abstract

The present study was carried out to elucidate the Hepato protective effect of dried mushroom against carbon tetrachloride (CCl4) induced hepato toxicity in liver and cytogenicity change in rats .A total of thirty adult male albino rats (150-200 g) were used in this study.Rats were divided into 6 groups each of 5 animals .Group one was kept as acontrol –ve and fed on basal ration only, while the two other groups were fed on basal ration mixed with grind dried mushroom at concentration 5 and 10 % for 30 successive days. Other three groups were subcutaneous injected of with CCl4 (0.1 ml/100 g b.wt. for two weeks) to induced liver fibrosis and cytogenicity changes .One of these group was left as a control +ve (subcutaneous injection of CCl4), whereas the other two groups were fed on basal ration mixed with grind dried mushroom at concentration 5 and 10 % for 30 successive days.

At the end of experimental period blood samples were collected from each rat for biochemical analysis and cytogenicity. Subcutaneous injection of CCl4 caused significant increase in serum levels of AST,ALT,ALP,creatinine,urea, triglyceride,glucose and total cholesterol LDL. Subcutaneous injection of CCl4 caused significantly increased in micronucleated polychromatic erythrocytes (MPCEs) and ratio of polychromatic to normochromatic erythrocytes (PCE / NCC).Feeding on ration mixed with dried mushroom and injected CCl4 caused significant decreases of MPCEs if compared with control +ve group and decrease of AST and ALT when compared with control +ve group and directed toward normal. The result showed that feeding on dried mushroom showed statistical decrease in micro nucleated polychromatic erythrocytes (MPCEs) and significantly increased in normo chromatic erythrocytes (NCE) compared with control –ve group. 

Keywords

Main Subjects

Files

Share

How to cite

Statistics