Effect of Sodium Benzoate on DNA Breakage and Antioxidant Enzymes in Male Rats

Document Type : Scientific and Research

Authors

1 Physiology ,Biology Department ,College of Science, Qassim Univ

2 Pharmacology ,Biochemistry Dep. AHRI,Egypt

3 Physiology,Biochemistry Dep. AHRI,Egypt

4 Biology Dep.,College of Science, Qassim Univ

5 Clinical nutrition, Nutrition Dep.,Research Institute of Ophthalmology,Egypt

Abstract

The present study was carried out to examine the effects of a food preservative sodium benzoate (SB) on DNA and some biochemical parameters. Because there is a controversy concerning the use of food preservatives, especially as they may have a carcinogenic effect. An adult fifteen males Sprague Dawley rats ,were used in the present experiment The rats were randomly divided into three groups, Group I: normal control group, Group II & III: treated groups were received SB at concentration of 2.5% and 4% for four weeks. At the end of the experiment the animals were sacrificed and samples were taken for biochemical analysis and Quantitative measure of DNA.
The aim of this study was to assess the effect of different concentrations of SB on the DNA breakage in liver cells and on some biochemical parameters in male rats. The obtained result showed that, quantitative measure of DNA significantly decreased in the amount of DNA/1g of liver when two concentrations of SB were added as compared with the control group.
Serum biochemical analysis, revealed significant elevation in total protein, albumin, SOD, GPX and AST at both doses 2.5% and 4% comparing to the control group. ALP was increased in 2.5% dose and decreased in 4% dose, ALT was increased in 4% dose. On the other hand, the body weight was significantly decreased in both treated groups as compared to the control group. Liver weight showed a significant increase in2.5% dose and decrease in 4% dose. 

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