Histological and biochemical effects of caffeine on bone of growing rats

El-Masry, Samir A; Badr, Osama M; Mansor, Magda A; Abdalla, Walid M

doi:10.21608/ejceh.2017.233111

Histological and biochemical effects of caffeine on bone of growing rats

Document Type : Scientific and Research

Authors

¹ Molecular Biology Department Sadat City University

² Animal Biotechnology Department, Sadat City University

³ Genetic Engineering and Biotechnology Institute (GEBRI), Sadat City University

⁴ Histology Department Faculty of Medicine, Minoufia University

10.21608/ejceh.2017.233111

Abstract

The present study has been carried out to evaluate the histological and biochemical effects of caffeine on bone of growing rats.
A total of 25 rats (8 weeks old) were divided randomly into three groups: Group 1(n = 5), serve as the control group, group 2 (n = 10), caffeine-low dose; group 3 (n =10), caffeine high dose. The caffeine was fed by gastric tube daily and the high dose of caffeine=10 mg/100 g body weight/day, and the low dose of caffeine=2.5 mg/100g body weight/day. Body weight was measured weekly. After sacrifice, blood samples were collected in tubes, and serum samples were separated for the determination of Ca, ALP, Zn and Mn. Femoral bones of rats were used for histopathological assessment. The results showed that serum Ca, Zn and Mn levels for high dose group is significantly lower than the low dose than the control group. The Alkaline phosphatase in low dose group is not significant smaller than the control group, but the high dose group has significantly elevation value than the control group. Thickness of epiphyseal plate especially proliferating and hypertrophic zones compared to control group was observed in the group which received low dose of caffeine. Section of diaphysis of low-dose treated group showed thinning out of the outer compact bone with multiple osteoporotic cavities in the bone matrix. The epiphyseal plates of caffeine-high dose showed disturbance of bone growth as revealed by presence of numerous clefts among rows of cartilage cells of the epiphyseal plate, between the proliferating and hypertrophic zone and between calcified and ossified zones.
Thus, it can be concluded that the oral administration of caffeine lead to a significant reduction in serum Ca, Zn and Mn; and a significant elevation in serum ALP together with a dose dependent histological changes in the bone of treated rats according to the increase of caffeine dose.

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