Hepatoprotective Effect of Jojoba Oil on DNA Damage and Antioxidant Enzymes Induced by Cadmium in Rats

Document Type : Scientific and Research

Authors

Biochem. Toxicol. & Feed Deff. Dep. AHRI

Abstract

The present investigation aimed to study the effect of the treated jojoba oil and / or ascorbic acid (vitamin C ) in rats exposed to cadmium chloride (Cdcl2) at adose 10 mg/ 100g.b.wt. for 30 successive days . Forty mature rats of an average body weight 150- 180 gm were used for the experiments . Fifteen rats were administered cadmium orally at dose 10mg/100gm.b.wt. for 30 successive days (for induced damage of DNA) . All rats divided into 8 equal groups of 5 rats each.Group1:served as control negative. Group2:fed on basal diet and administered cadmium orally for 30 days(control positive).Group3:fed on basal diet mixed with treated jojoba oil in ratio 2.5%. Group4:fed on basal died and administered orally vitamin C. Group5: fed on basal diet mixed with treated jojoba oil in ratio 2.5% and administered vitamin C Goup6(pretreated with cadmium) fed on basal diet mixed with treated J.O. in ratio 2.5% for 30 days. Group7(pretreated with cadmium) fed on basal diet and administered vitamin C orally for 30 days.Group8(pretreated with cadmium) fed on basal diet mixed with treated J.O. in ratio 2.5% and administered vitamin C orally for 30 days.
The obtained results showed that administration of treated jojoba oi land/or vitamin C to cadmium chloride intoxicated rats induced significant increase in the amount of DNA / gm of spleen ,decrease in the percentage of MPCE and increase the frequency of NCE .
Cadmium exposure increased the production of reactive oxygen species (ROS) and altered the levels of oxidative stress related biomarkers of toxicity. Results revealed that cadmium significantly increased catalase enzyme activity and lipid peroxidation concentration (MDA) while reduced glutathione was decreased in rat sera. Furthermore, cadmium exposure were associated with depletion of serum levels of vitamin C, E, A and β- carotene. As compared to the results obtained in control groups the study showed that a lower concentration of serum proteins and albumin were accompanied by decreased globulin alpha 1 and beta along with an increased gamma 2 globulin; and the activity of serum GGT, LDH, ALP and concentrations of cholesterol, and triglyceride,were higher. Moreover, jojoba oil and vitamin C have ameliorated the cadmium-induced toxicity by improving antioxidant markers. The results of this investigation demonstrated that pretreatment with jojoba oil and vitamin C have the  potentials to countermeasure the immunosuppressive, biochemical alteration as well as oxidative damage induced by cadmium in rats. 

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