Protective effect of some antioxidant compound on albino rats intoxicated with aflatoxin

Document Type : Scientific and Research

Authors

1 Departments of Biochemistry, Animal Health Research Institute, DokkiGiza; Veterinary Laboratory, Tanta, El-gharbia Governorate, Egypt

2 Departments of Mycology, Animal Health Research Institute, DokkiGiza; Veterinary Laboratory, Tanta, El-gharbia Governorate, Egypt

3 Departments of Pathology, Animal Health Research Institute, DokkiGiza; Veterinary Laboratory, Tanta, El-gharbia Governorate, Egypt.

Abstract

Mycotoxins are unavoidable contaminants of animal and human feed and food respectively. This study was aimed to investigate the effect of Vitamin E, Vitamin C and Selenium Supplementation to drink water against aflatoxins B1 on the various serum parameters , liver tissue antioxidant and the associated pathological changes. Thirty five male albino rats weighing 120-150 g were used in the present study. Group1 (G1): rats served as control , Group2 (G2) orally administrated aflatoxin B1 dissolved in propylene glycol per Os as a dose of 50µg/kg body weight /week, for 10 weeks . Group3-7 : administrated with aflatoxin B1 50µg/rat/week for 10 weeks . These groups were treated as Group3 (G3):was supplemented with selenium (as sodium selenite was used as 4mg/l). Group4 (G4):was supplemented with vitamin E as 1000mg/l. Group5 (G5):was supplemented with vitamin E plus selenium. Group6 (G6): was supplemented with vitamin C as 1000mg/l. Group7 (G7):was supplemented with vitamin C plus selenium. The results showed significant elevation in the liver and kidney functions, alpha globulin and beta globulin and decrease in concentrations of serum total protein, albumin, and gamma globulin together with A/G ratio. Lipid peroxidase as MDA levels were shown after AFB1 treatment. pathological changes of liver in aflatoxicated rats was discussed briefly. It can be concluded that the hepatotoxicity induced by aflatoxin B1 seemed to be modulated effectively by the simultaneous use of antioxidants: vitamin E or C alone or incombination, with selenium. Moreover, they are more efficient modulating the biochemical alteration , liver antioxidant enzymatic system and pathological changes.

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